Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by EDTA and EGTA at up to 20 mM | Morchella importuna |
Application | Comment | Organism |
---|---|---|
agriculture | since the enzyme degrades phytate in feed materials efficiently under low temperature and weak acidic conditions, which are common for aquacultural application, it might be a promising candidate as a feed additive enzyme | Morchella importuna |
Cloned (Comment) | Organism |
---|---|
gene phyA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant overexpression in Pichia pastoris strain GS115 | Morchella importuna |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | slight inhibition | Morchella importuna | |
Ba2+ | - |
Morchella importuna | |
Cu2+ | slight inhibition | Morchella importuna | |
Fe2+ | slight inhibition | Morchella importuna | |
Fe3+ | - |
Morchella importuna | |
Hg2+ | - |
Morchella importuna | |
Mn2+ | slight inhibition | Morchella importuna | |
Pb2+ | - |
Morchella importuna | |
SDS | 90% inhibition at 1%, complete inhibition at 10%, 10% inhibition at 0.1% | Morchella importuna | |
Urea | 40% inhibition at 100 mM, complete inhibition at 5 M | Morchella importuna | |
Zn2+ | slight inhibition | Morchella importuna |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | no signal peptide is identified | Morchella importuna | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61089 | - |
mass spectrometry | Morchella importuna |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Morchella importuna | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Morchella importuna SCYDJ1A1 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Morchella importuna | A0A2H4W727 | - |
- |
Morchella importuna SCYDJ1A1 | A0A2H4W727 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Morchella importuna | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2384.6 | - |
purified enzyme, pH 6.5, 25°C | Morchella importuna |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal | Morchella importuna | ? | - |
? | |
additional information | the enzyme also shows moderate activity with 4-nitrophenyl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1,6-bisphosphate, and glucose 6-phosphate, and low activity with AMP, ADP, and ATP. The enzyme cleaves phytate in soybean meal, rapeseed meal, and corn meal | Morchella importuna SCYDJ1A1 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Morchella importuna | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Morchella importuna SCYDJ1A1 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 61090, sequence calculation, 1 * 61089, mass spectrometry, 1 * 60000, SDS-PAGE | Morchella importuna |
Synonyms | Comment | Organism |
---|---|---|
MiPhyA | - |
Morchella importuna |
PhyA | - |
Morchella importuna |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Morchella importuna |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
30 | over 85% of maximal activity at 15-25°C, maximal activity at 25°C, sharp drop inactivity at above 30°C, over 50% of maximal activity at 0-5°C | Morchella importuna |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
purified enzyme, pH 6.5, 180 min, 50% activity remaining | Morchella importuna |
45 | - |
purified enzyme, pH 6.5, 50% activity remaining after 120 min, 20% after 180 min | Morchella importuna |
65 | - |
purified enzyme, pH 6.5, 50% activity remaining after about 20 min, 10% after 60 min, inactivation after 120 min | Morchella importuna |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Morchella importuna |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 7.5 | activity range, profile overview. sharp drop in activity above pH 6.5 | Morchella importuna |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
purified enzyme, 25°C, about 50% activity remaining after about 50 min, inactivation after 180 min | Morchella importuna |
6.5 | - |
purified enzyme, 25°C, 240 min, 40% activity remaining | Morchella importuna |
11 | - |
purified enzyme, 25°C, about 50% activity remaining after about 50 min, inactivation after 180 min | Morchella importuna |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the histidine acid phosphatase (HAP) family, it possesses typical conserved motifs of HAP phytases: RHGERFP in AA125-131 and HD in AA394-395 | Morchella importuna |